EN 14735:2005 – Characterization of waste – Preparation of waste samples for ecotoxicity tests
1 Scope
This European Standard describes the necessary steps to be performed before carrying out ecotoxicity tests on wastes. The purpose of this European Standard is to provide guidance on the taking of the sample, transport, storage of wastes and to define preparation, for the determination of ecotoxicological properties of wastes under the conditions specified in this European Standard by biological testing either as raw wastes or water extracts from wastes. Sample preparation for other applications (e.g. assessment of waste effects on aquatic and terrestrial organisms in a disposal scenario) is not considered.
Specifying a test battery to characterize ecotoxicological properties of wastes is not in the scope of this European Standard.
This European Standard is applicable to solid and liquid wastes.
2 Normative references
The following referenced documents are indispensable for the application of this European Standard.
For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
prEN 14899, Characterization of waste – Sampling of waste materials – Framework for the preparation and application of a Sampling Plan
EN 12457-2:2002, Characterization of waste – Leaching – Compliance test for leaching of granular waste materials and sludges – Part 2: One stage batch test at a liquid to solid ratio of 10 l/kg for materials with particle size below 4 mm (without or with size reduction)
EN ISO 5667-3, Water quality – Sampling – Part 3: Guidance on the preservation and handling of water samples (ISO 5667-3:2003)
ISO 10390, Soil quality – Determination of pH
ISO 11268-1, Soil quality – Effects of pollutants on earthworms (Eisenia fetida) – Part 1: Determination of acute toxicity using artificial soil substrate 1)
ISO 11465, Soil quality – Determination of dry matter and water content on a mass basis – Gravimetric method
ISO 14238:1997, Soil quality – Biological methods – Determination of nitrogen mineralization and nitrification in soils and the influence of chemicals on these processes
3 Terms and definitions
For the purposes of this European Standard, the following terms and definitions apply.
3.1
dilution medium
liquid or solid used for the preparation of control vessels and the preparation of test mixtures
3.2
ecotoxicological properties
potential adverse effects to biological systems which a waste has an inherent capacity to cause
3.3
eluate
solution recovered from a leaching test
[EN 12457-2:2002]
3.4
granular waste
waste not being monolithic, nor a liquid, a gas or a sludge
[EN 12457-2:2002]
3.5
laboratory sample
sample or subsample(s) sent to or received by the laboratory (IUPAC definition)
EN 14735:2005 – Characterization of waste – Preparation of waste samples for ecotoxicity tests